{"id":8957,"date":"2024-05-28T15:43:27","date_gmt":"2024-05-28T19:43:27","guid":{"rendered":"https:\/\/www.iucpq.ca\/?page_id=8957"},"modified":"2025-04-23T11:18:52","modified_gmt":"2025-04-23T15:18:52","slug":"microscopy","status":"publish","type":"page","link":"https:\/\/www.iucpq.ca\/en\/research-center\/platforms\/microscopy\/","title":{"rendered":"Microscopy"},"content":{"rendered":"\n<section class=\"wp-block-stereo-section-bloc section-block-normal\"><div class=\"wp-block-stereo-section__container\"><div class=\"wp-block-stereo-section__col is-layout-flow wp-block-stereo-section-bloc-is-layout-flow\">\n<h2 class=\"wp-block-heading\">Microscopy Platform<\/h2>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<h4 class=\"wp-block-heading\">High-quality microscopes and a workstation for image processing and quantification are available to the scientific community through the <em>Centre de recherche<\/em>.<\/h4>\n<\/blockquote>\n\n\n\n<p>One microscope (widefield) captures colour and fluorescence images and a second microscope allows for confocal acquisitions in fluorescence. Both systems are equipped for real-time acquisitions (live mode) with a motorized stage.<\/p>\n<\/div><\/div><\/section>\n\n\n\n<section class=\"wp-block-stereo-section-bloc section-block-normal -section-alt\"><div class=\"wp-block-stereo-section__container\"><div class=\"wp-block-stereo-section__col is-layout-flow wp-block-stereo-section-bloc-is-layout-flow\">\n<h2 class=\"wp-block-heading\">Equipment and Services<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">Confocal System<\/h3>\n\n\n\n<p>LSM800 microscope, Zeiss Axio Observer Z1 motorized inverted microscope<\/p>\n\n\n\n<figure data-module=\"Figcaption\" class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1200\" height=\"800\" src=\"https:\/\/www.iucpq.ca\/app\/uploads\/2024\/07\/laboratoire-microscopie.png\" alt=\"\" class=\"wp-image-7717\" style=\"width:461px;height:auto\" srcset=\"https:\/\/www.iucpq.ca\/app\/uploads\/2024\/07\/laboratoire-microscopie.png 1200w, https:\/\/www.iucpq.ca\/app\/uploads\/2024\/07\/laboratoire-microscopie-1024x683.png 1024w, https:\/\/www.iucpq.ca\/app\/uploads\/2024\/07\/laboratoire-microscopie-500x333.png 500w, https:\/\/www.iucpq.ca\/app\/uploads\/2024\/07\/laboratoire-microscopie-768x512.png 768w\" sizes=\"auto, (max-width: 1200px) 100vw, 1200px\" \/><\/figure>\n\n\n\n<p>Possible acquisitions:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Fluorescence from a confocal mode<\/li>\n\n\n\n<li>Transmitted light image acquisition<\/li>\n\n\n\n<li>Imaging of living cells<\/li>\n\n\n\n<li>Z-stacks<\/li>\n\n\n\n<li>Time-Lapse<\/li>\n\n\n\n<li>Mosaics<\/li>\n<\/ul>\n\n\n\n<p>This system provides:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Four solid-state laser lines: 405, 488, 561, 640 nm with a line scan rate of eight frames per second at 512 \u00d7 512 pixels<\/li>\n\n\n\n<li>Spectral sensing with two GaAsP detectors<\/li>\n\n\n\n<li>Third \u201cAiryscan\u201d-type detector for a \u201csuper resolution\u201d at 140 nm in X and Y, and at 400 nm in Z<\/li>\n\n\n\n<li>External transmitted light detector system for superposition of DIC and confocal-contrast images<\/li>\n\n\n\n<li>Objective lens: 10x (0.25 NA), 20x (0.8 NA), 40x (0.6 NA, long working distance), 40x (1.4 NA, oil), 63x (1.4 NA, oil), 63x (0.85 NA, air).<\/li>\n\n\n\n<li>Equipped for experiments on living cells (temperature control, CO<sub>2<\/sub>, humidity)<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Widefield System<\/h3>\n\n\n\n<p>Motorized inverted microscope, Zeiss Axio Observer Z1<\/p>\n\n\n\n<figure data-module=\"Figcaption\" class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"224\" height=\"157\" src=\"https:\/\/www.iucpq.ca\/app\/uploads\/2024\/07\/microscope-inverse-motorise.png\" alt=\"\" class=\"wp-image-7715\"\/><\/figure>\n\n\n\n<p>Possible acquisitions:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Colour images<\/li>\n\n\n\n<li>Fluorescence images<\/li>\n\n\n\n<li>Differential interference contrast (DIC)<\/li>\n\n\n\n<li>Imaging of living cells<\/li>\n\n\n\n<li>Z-stacks<\/li>\n\n\n\n<li>Time-lapse<\/li>\n\n\n\n<li>Mosaics<\/li>\n<\/ul>\n\n\n\n<p>This system provides:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>DIC, brightfield<\/li>\n\n\n\n<li>Fluorescence: Dapi (filter 49), GFP (filter 38), Cy3 (filter 43), mRFP (filtre 63), Cy5 (filter 50), Quad filter (Dapi, FITC, Texas Red, Cy5)<\/li>\n\n\n\n<li>Objectives: 10x (0.25 NA), 20x (0.4 NA, long working distance), 20x (0.8 NA), 40x (0.6 NA, long working distance), 40x (1.4 NA, oil), 63x (1.4 NA, oil), 100x (1.46 NA, oil)<\/li>\n\n\n\n<li>Illumination: Hummingbird (LED) and metal halide lamps, LED lighting for transmitted and polarized light<\/li>\n\n\n\n<li>Cameras: Axiocam 105 (colour; 47 fps, 5 MP) and Axiocam 506 (monochrome; 51 fps, 6 MP)<\/li>\n\n\n\n<li>Definite focus<\/li>\n\n\n\n<li>ApoTome.2 (structured illumination for acquiring optical sections of 1 Airy unit)<\/li>\n\n\n\n<li>Equipped for experiments on living cells (temperature control, CO<sub>2<\/sub>, humidity)<\/li>\n<\/ul>\n<\/div><\/div><\/section>\n\n\n\n<section class=\"wp-block-stereo-section-bloc section-block-normal\"><div class=\"wp-block-stereo-section__container\"><div class=\"wp-block-stereo-section__col is-layout-flow wp-block-stereo-section-bloc-is-layout-flow\">\n<h2 class=\"wp-block-heading\">Request for the use<\/h2>\n\n\n\n<p>The equipment can be found in room Y4397.<\/p>\n\n\n\n<p>Both systems are operational according to the user-pay principle. Training is mandatory for new users. It includes introduction to the system (widefield or confocal) as well as assistance during the first images collected. It is also possible to get<\/p>\n\n\n\n<p>Reservations are made via the CRIUCPQ intranet.<\/p>\n\n\n\n<p>In order to make a service request or a reservation without going through the intranet or for any further information, please contact :<\/p>\n\n\n<div class=\"o-section-gutenberg\">\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-28f84493 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<div >\n\t\n<div class=\"c-box u-box \">\n\t<div class=\"c-box__content \">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"c-box__content__infos\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<h3 class=\"c-box__title\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span>Kerstin Bellmann<\/span>\n\t\t\t\t\t\t\t\t\t\t\t<\/h3>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<hr>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"o-flex -flex-gap-xxs -flex-align-center\">\n\t\t\t\t\t\t\t<svg aria-hidden=\"true\" focusable=\"false\" class=\"o-ico -ico-sm\">\n\t\t<use xlink:href=\"https:\/\/www.iucpq.ca\/app\/themes\/baserock\/assets\/img\/sprite.svg?v=3544c6af9f0b59e36b#tel\"><\/use>\n\t<\/svg>\n\t\t\t\t\t\t\t<a href=\"tel:+14186568711,3782\" target=\"_blank\">\n\t\t\t\t\t\t\t418 656-8711, ext 3782\t\t\t\t\t\t<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"o-flex -flex-gap-xxs -flex-align-center\">\n\t\t\t\t\t\t\t<svg aria-hidden=\"true\" focusable=\"false\" class=\"o-ico -ico-sm\">\n\t\t<use xlink:href=\"https:\/\/www.iucpq.ca\/app\/themes\/baserock\/assets\/img\/sprite.svg?v=3544c6af9f0b59e36b#mail\"><\/use>\n\t<\/svg>\n\t\t\t\t\t\t\t<a href=\"mailto:kerstin.bellmann@criucpq.ulaval.ca\" target=\"_blank\">\n\t\t\t\t\t\t\tkerstin.bellmann@criucpq.ulaval.ca\t\t\t\t\t\t<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n<\/div>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\"><\/div>\n<\/div>\n<\/div><\/div><\/div><\/section>\n\n\n\n<section class=\"wp-block-stereo-section-bloc section-block-normal -section-alt\"><div class=\"wp-block-stereo-section__container\"><div class=\"wp-block-stereo-section__col is-layout-flow wp-block-stereo-section-bloc-is-layout-flow\">\n<h2 class=\"wp-block-heading\">Fees<\/h2>\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"https:\/\/www.iucpq.ca\/app\/uploads\/2024\/09\/Fee-Schedule-Microscopy-20240701.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Fee Schedule<\/a><\/div>\n<\/div>\n<\/div><\/div><\/section>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":0,"parent":10541,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"_relevanssi_hide_post":"","_relevanssi_hide_content":"","_relevanssi_pin_for_all":"","_relevanssi_pin_keywords":"","_relevanssi_unpin_keywords":"","_relevanssi_related_keywords":"","_relevanssi_related_include_ids":"","_relevanssi_related_exclude_ids":"","_relevanssi_related_no_append":"","_relevanssi_related_not_related":"","_relevanssi_related_posts":"","_relevanssi_noindex_reason":"","footnotes":""},"class_list":["post-8957","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.iucpq.ca\/en\/wp-json\/wp\/v2\/pages\/8957","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.iucpq.ca\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.iucpq.ca\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.iucpq.ca\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.iucpq.ca\/en\/wp-json\/wp\/v2\/comments?post=8957"}],"version-history":[{"count":9,"href":"https:\/\/www.iucpq.ca\/en\/wp-json\/wp\/v2\/pages\/8957\/revisions"}],"predecessor-version":[{"id":17914,"href":"https:\/\/www.iucpq.ca\/en\/wp-json\/wp\/v2\/pages\/8957\/revisions\/17914"}],"up":[{"embeddable":true,"href":"https:\/\/www.iucpq.ca\/en\/wp-json\/wp\/v2\/pages\/10541"}],"wp:attachment":[{"href":"https:\/\/www.iucpq.ca\/en\/wp-json\/wp\/v2\/media?parent=8957"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}